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KMID : 0364819870250010057
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Korean Journal of Microbiology
1987 Volume.25 No. 1 p.57 ~ p.66
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E. coli Mutants sensitive to Alkylating agents and their Complementary Gene
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Jung, Seun-Ho/정선호
Han, Bo-Mie/Yang, Chul-Hak/한범희/양철학
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Abstract
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1
Mutants of E. coli which showed increased sensitivity to MMS(methyl methane sulfonate) were isolated by MNNG mutagenesis and characterized by enzymatic assay, survival of simple alkylating agents and host-cell reactivation. E.coli mutant, 5-62, which showed absolute deficiency in 3-methyladenine DNA glycosylase II activity and had low capability of reactivating MMS-treated phage charon 35 was very sensitive to MMS and MNNG. MMS´ gene which confered resistance to the lethal effects of MMS was cloned in 5-62 strain.
5-62 mutants carrying recombinant plasmid, pMRG 1, which acquired resistance to the lethal effects of MMS had normal sensitivity to MNNG. Resistance to MMS was somewhat increased after they were treated with 0.5 Mg MNNG/ml for 2 hours at 37°C. Although recombinant plasmid, pMRG 1, did not complement alk A mutation in 5-62 and ada mutation in 1-27 mutant, mutants transformed with this plasmid showed more capability of reactivating MMS treated phage than mutants.
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